Documents document view page perkin elmer janus liquid. Utilizing a dishshaped acoustic transducer and computercontrolled electronics. Chiller for sseries, eseries and leseries afa ultrasonicators. Consult the manufacturers recommendations for use of other covaris instruments or sample holders to achieve the same target dna fragment size. Dna shearing with microtubes covaris water conditioning system. Two parameters were mod ulated to optimize the yields of gdna fragments in the size range of interest. This kit allows users to routinely verify the performance of their covaris. See the question what are the settings for the s220 and m220 covaris models.
The company refers to this process as adaptive focused acoustic energy. It is ideal for the production of dna libraries for nextgeneration sequencing. Related documents document title document number raindance 3kb shearing pr. This manual includes sections on the operating instructions, maintenance guidelines, and troubleshooting tips.
Covaris are your leading technical specialists in physical asset management, analytics, maintenance and change management. This instrument can used by customers for precisely shearing dna in preparation for illumina sequencing and other applications. S220 x user manual page 4 warranty when used in accordance with written instruction and under normal operating conditions, the covaris instruments are guaranteed to be free of defects in material and workmanship for one 1 year from the date of original delivery by an authorized representative. The e220 can be operated as a standalone unit or easily integrated as part of an automated laboratory system. The covaris m220 ultrasonicator provides mechanical shearing of dna to generate tight, highly reproducible fragment distributions. Covaris s220 ultrasonicator user operated holders and tubes are available for 50ul to 2ml sample volumes. System degas pumps should be run in advance of and during afa treatments, as detailed in instrument user manuals. Similarly, insufficient degas levels within the bath may result in poor acoustic coupling and thereby shift the mean fragment size. This instrument is designed to provide researchers an integrated standalone tool to be used in a laboratory environment. I have been working at covaris fulltime more than 8 years pros. Unpacking and instructions covaris sseries youtube.
Used covaris e220 focused ultrasonicator sample preparer. This manual contains operation and maintenance instructions for the covaris e and leseries instruments the e220, e220x and le220. Recommended settings are subject to change without notice. The target fragment size range can be chosen between 150 bp and 5 kb. The e220 focusedultrasonicator enables multisample batch preparation, capable of processing a wide range of sample types and volumes. Fragmentation was performed in 96well format according to manufacturer specifications 14. Optimized shearing protocols are available for fragment sizes from 150 to 5,000 bp. Tissue chip protocols, cultured cells chip protocols, msds information. The e220 evolution and the covaris process the e220 evolution focusedultrasonicators are based on covaris adaptive focused acoustics afa technology.
The covaris e220 enables highthroughput sample sonication and processes from one to 96 samples in a 96well plate or in individual glass microtubes. Covaris assumes no liability regarding the use of a protocol for applications in which it is not intended or with use of non covaris certified consumables. Optimization of covaris settings for shearing bacterial. Covaris e210 focused ultrasonicator wchiller circulator predecessor to e220 10808 duration. Covaris provides tools and technologies to improve preanalytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process. The versatility of the eseries focusedultrasonicator makes it possible to bring the advantages of afa to numerous biological and chemical applications including, dna and chromatin shearing, tissue homogenization, cell lysis, compound. Covaris e220 focused ultrasonicator multisample w vwr mm7. The e220 focusedultrasonicators are versatile instruments engineered for preanalytical sample processing by adaptive focused acoustics. The covaris focused sonicators are the standard equipment to reproducibly shear nucleic acid and chromatin in an unbiased fashion for highthroughput.
Covaris le220 focused ultrasonicator bostonind 873. The e220 may be programed to process from 1 to 96 samples in a single batch. The covaris e220 evolution instrument available at ggbc is mostly used for dna shearing application. The e220 can be operated as a standalone unit, or easily integrated as part of an automated laboratory system. Company covaris, inc price get quote get quote for this product. The covaris instrument works by using acoustic energy wave. Covaris e210 user manual uncheck hide file extensions for known file types my boss did lose a lot of money by losing the contract, said dogg. Set up the covaris s2 or e210 at the appropriate temperature. Dna shearing with e220 focusedultrasonicator covaris. Each strip contains 8 microtube and allows great flexibility when processing samples in varying batch sizes. With over 18 years experience in consultation and a strong background in industry, we can progress your projects and provide solutions. User manual an automated, batch system for high performance sample preparation using adaptive focused acoustic energy. It contains background information essential to the proper use and care of this equipment.
Each strip contains 8 microtube and allows great flexibility when processing samples in. Covaris covaris e110 focused ultrasonicator covaris e220 focused ultrasonicator multisample w vwr mm7 recirculating chiller. The versatility of the eseries focusedultrasonicator makes it possible to bring the advantages of afa to numerous biological and chemical applications including, dna and chromatin shearing, tissue homogenization, cell lysis. Preparation using adaptive focused acoustic afa energy. If youre a talented person, that can get things done and can bring value to the company, then covaris is the best place to work for you. Table 10 shear settings for covaris eseries instrument sonolab software v7 or later. Instrument and software developed for highperformance sample. Mechanical shearing for next generation sequencing ngs dnarna extraction from formalinfixed, paraffinembedded. Please note while using the e220 and e220evolution, the intensifier.
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